pUC19 - Wikipedia
WEBpUC19 is one of the most widely used vector molecules as the recombinants, or the cells into which foreign DNA has been introduced, can be easily distinguished from the non-recombinants based on color differences of colonies on growth media. pUC18 is similar to pUC19, but the MCS region is reversed.
pUC19 Sequence and Map - SnapGene
WEBpUC19 Sequence and Map. Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. The MCS is reversed in pUC18. Sequence Author: New England Biolabs. Open in SnapGene. Try SnapGene for Free. Download Plasmid. | Download SnapGene Viewer. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets.
Addgene: pUC19
WEBpUC19. (Plasmid #50005) Print. Enlarge. View all sequences. Purpose. (Empty Backbone) pUC cloning vector. Depositing Lab. Joachim Messing. Publication. Norrander et al Gene. 1983 Dec;26 (1):101-6. ( How to cite ) Sequence Information. Sequences (3) Ordering. This material is available to academics and nonprofits only. Backbone. Vector backbone.
pUC19 Vector | NEB
WEBpUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).
pUC19 Vector- Definition, Structure, Sites, Applications - Microbe …
WEBAug 3, 2023 · pUC19 Vector- Definition, Structure, Sites, Applications. August 3, 2023 by Nidhi Abhay Kulkarni. Edited By: Sagar Aryal. pUC plasmids were first developed by Joachim Messing and his co-workers. It is a commonly used cloning vector in the bacteria E. coli . pUC19 is 2686 bp in length.
pUC18 and pUC19 vectors - Takara Bio
WEBThe pUC18 and pUC19 plasmids are suitable for dideoxy DNA sequencing using M13 primers. To sequence pUC18 and pUC19 plasmids containing large DNA insertions, utilize the Deletion Kit for Kilo-Sequencing (Cat. # 6030). More Information. Applications. Cloning and expression of target genes. DNA sequencing using M13 primers.
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pUC19 - NEB
WEBpUC19 is a small, high-copy number E. coli plasmid cloning vector containing portions of pBR322 and M13mp19 (1). It con-tains the pMB1 origin of replication from pBR322, but it lacks the rop gene and carries a point mutation in the RNAII transcript. (G 2975 in pBR322 to A 1308 in pUC19; 2).
Addgene: pUC19 Sequences
WEBpUC19. Sequences. pUC19 Sequences (3) Addgene Sequences: Full (1) Partial (2) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). Analyze Sequence. GenBank. SnapGene. File …
pUC19L Sequence and Map - SnapGene
WEBDownload pUC19L.dna file. Download Plasmid. Open in SnapGene. SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures. Fast accurate construct design for all major molecular cloning techniques. Validate sequenced constructs using powerful alignment tools.
pUC19 DNA - Thermo Fisher Scientific
WEBThermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation. Highlights • Purified by chromatography using proprietary patented technology
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